Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

Abstract
The nucleotide sequence of cloned cDNA for preproenkephalin from bovine adrenal medulla indicates that the precursor protein contains four copies of Met-enkephalin and one copy each of Leu-enkephalin, Met-enkephalin-Arg6 -Phe7 and Met-enkephalin-Arg6 -Gly7 -Leu8, a previously undetected opioid peptide. The enkephalin and extended enkephalin sequences are each bounded by paired basic amino acid residues. Preproenkephalin may represent a multi-hormone precursor, like the corticotropin-β-lipotropin precursor.