In Vitro Cultivation of the Exoerythrocytic Stage of Plasmodium Berghei in a Hepatoma Cell Line

Abstract
When inoculated with sporozoites of Plasmodium berghei, a line of hepatoma cells (HepG2-A16) derived from human liver supports the complete asexual developmental cycle of the exoerythrocytic stage. Parasites were shown to resemble parasites in vivo in hepatocytes. Subinoculation of merozoites into mice induced a red blood cell infection.