TRANSIENT CHANGES IN EXTRINSIC FLUORESCENCE OF NERVE PRODUCED BY ELECTRIC STIMULATION
- 1 December 1969
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (4), 1362-1368
- https://doi.org/10.1073/pnas.64.4.1362
Abstract
Nerves stained with one of the following fluorochromes were found to respond to electric stimulation with transient changes in the fluorescent light: acridine orange, acridine yellow, 8-anilinonaphthalene-1-sulfonate, auramine O, 1-dimethylaminonaphthalene-5-sulfonyl chloride, fluorescein isothiocyanate, LSD, pyronin B, pyronin Y, rhodamine B, rhodamine G, rose bengal, and 2-toluidinylnaphthalene sulfonate. The properties of covalently bonded fluorochromes in the nerve and the behavior of fluorochromes introduced into the axon interior were studied. By measuring fluorescence polarization, direct evidence was obtained showing a decrease in intramembrane viscosity during the action potential.Keywords
This publication has 5 references indexed in Scilit:
- Changes in fluorescence, turbidity, and birefringence associated with nerve excitation.Proceedings of the National Academy of Sciences, 1968
- Light Scattering and Birefringence Changes during Nerve ActivityNature, 1968
- AGGREGATION OF DYES BOUND TO POLYANIONSProceedings of the National Academy of Sciences, 1959
- Rotational Brownian Motion and Polarization of the Fluorescence of SolutionsAdvances in protein chemistry, 1953