Isolation of a new gene (SW A2) encoding an α‐amylase from Schwanniomyces occidentalis and its expression in Saccharomyces cerevisiae
- 11 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (1), 41-44
- https://doi.org/10.1016/0014-5793(91)80245-x
Abstract
A new gene (SW A2) encoding a secretory α-amylase activity from Schwanniomyces occidentalis has been cloned from this year and then expressed in Saccharomyces cerevisiae. Both Sw. occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNA carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of α-amylase activity is drastically expressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.Keywords
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