Determination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay.
- 1 September 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (9), 1694-1699
- https://doi.org/10.1093/clinchem/23.9.1694
Abstract
Two immunochemical methods for determination of thyroxine-binding globulin in human serum were developed, in which the purified globulin and monospecific antiserum to it are used. One method, based on radial immunodiffusion, has good precision and values for analytical recovery. Reference values obtained for men were 9.8-17.8 mg/liter and for women 11.3-20.5 mg/liter. The sex-related difference was significant. The other method is based on radioimmunoassay, with use of an iodinated acylating agent for the labeling of thyroxine-binding globulin. The relative merits of the two methods are discussed.This publication has 2 references indexed in Scilit:
- Preparation of a monospecific antiserum to thyroxine binding globulin for its quantitation by rocket immunoelectrophoresisClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- THYROXINE-PROTEIN INTERACTIONS .V. ISOLATION AND CHARACTERIZATION OF A THYROXINE-BINDING GLOBULIN FROM HUMAN PLASMA1968