Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase.
- 1 June 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (6), 2054-2057
- https://doi.org/10.1073/pnas.72.6.2054
Abstract
Two mutants with specific defects in cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1) have been isolated from cultures of Saccharomyces cerevisiae exposed to the mutagens ethyl-methane sulfonate and Mn++. The mutations have been shown to be extranuclear by two criteria. The phenotype persists in diploids formed by a cross with a p-o strain of yeast of the opposite mating type. Tetrad analysis indicates a non-Mendelian segregation (4:0 and 0:4) of the mutations. Both mutants show a total absence of cytochrome oxidase activity and of spectral cytochromes a and as. One of the mutants has been shown to be missing a polypeptide synthesized by mitochondria. The migration of this protein on polyacrylamide gels corresponds to the highest-molecular-weight subunit of cytochrome oxidase.Keywords
This publication has 15 references indexed in Scilit:
- Cytochrome c oxidase from bakers' yeast. II. Site of translation of the protein components.1973
- Inhibition of the Assembly of Cytochrome Oxidase in Neurospora crassa by ChloramphenicolEuropean Journal of Biochemistry, 1972
- Cycloheximide Resistant Incorporation of Amino Acids into a Polypeptide of the Cytochrome Oxidase of Neurospora crassaEuropean Journal of Biochemistry, 1971
- Assembly of the mitochondrial membrane system. IV. Role of mitochondrial and cytoplasmic protein synthesis in the biosynthesis of the rutamycin-sensitive adenosine triphosphatase.1971
- The petite mutation in YeastJournal of Molecular Biology, 1970
- Mitochondrial genetics. I. Methodology and phenomenology.1970
- Assembly of the mitochondrial membrane system. I. Characterization of some enzymes of the inner membrane of yeast mitochondria.1969
- The Mutational Alteration of the Primary Structure of Yeast Iso-1-cytochrome cJournal of Biological Chemistry, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- La synthèse adaptive des cytochromes chez la levure de boulangerieBiochimica et Biophysica Acta, 1950