Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements.

  • 1 July 1968
    • journal article
    • Vol. 3 (6), 571-84
Abstract
The quantitative evaluation of the reactivity in human PHA-stimulated lymphocyte cultures was studied. A technique for the measurement of DNA synthesis by means of thymidine-2-14C incorporation was standardized and its reproducibility determined. Using this method a number of variables in culture conditions were investigated. The results obtained are discussed.