Yeast tRNAAsp recognition by its cognate class II aminoacyl-tRNA synthetase
- 1 March 1993
- journal article
- Published by Springer Nature in Nature
- Vol. 362 (6416), 181-184
- https://doi.org/10.1038/362181a0
Abstract
Aminoacyl-RNA synthetases can be divided into two classes according to structural features inferred from sequence alignments. This classification correlates almost perfectly with the attachment of the amino acid to the 2'-OH (class I) or 3'-OH (class II) group of the terminal adenosine. Six subgroups of higher homology can be inferred from sequence analysis. The five aminoacyl-tRNA synthetases whose crystal structures are known (MetRS, TyrRS and GlnRS in class I, SerRS and AspRS in class II) belong to different subgroups. Two of them, GlnRS and AspRS, have been cocrystallized with their cognate tRNA. AspRS, like six other members of class II, is an alpha 2 dimer. Yeast tRNA(Asp) exhibits five identity determinants: the three anticodon bases, the discriminator base G73 and the base pair G10-U25. We report here that the refined crystal structure of AspRS complexed with tRNA(Asp) at 2.9 A resolution reveals three regions of contact, each involving a domain of AspRS and at least one identity determinant of tRNA(Asp). The mode of binding of the acceptor stem of tRNA(Asp) by AspRS can be generalized to class II aminoacyl-tRNA synthetases, whereas the deciphering of the anticodon, which involves a large conformational change of the loop and the formation of a bulge, is more specific to the aspartic system.Keywords
This publication has 28 references indexed in Scilit:
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Cytoplasmic aspartyl‐tRNA synthetase from Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1991
- Crystallographic study at 2·5 Å resolution of the interaction of methionyl-tRNA synthetase from Escherichia coli with ATPJournal of Molecular Biology, 1990
- Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifsNature, 1990
- Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetaseGene, 1989
- Structure of tyrosyl-tRNA synthetase refined at 2.3 Å resolutionJournal of Molecular Biology, 1989
- Crystallographic refinement by simulated annealingJournal of Molecular Biology, 1988
- Crystallographic refinement of yeast aspartic acid transfer RNAJournal of Molecular Biology, 1985
- Specific Sequence Homology and Three-Dimensional Structure of an Aminoacyl Transfer RNA SynthetaseScience, 1984
- Hydrolytic action of aminoacyl-tRNA synthetases from Baker's yeast: "chemical proofreading" preventing acylation of tRNAIle with misactivated valineBiochemistry, 1976