Plant regeneration from pea protoplasts via somatic embyogenesis
- 1 October 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (7), 379-382
- https://doi.org/10.1007/bf00270073
Abstract
Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA3 — containing medium.Keywords
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