Capillary Electrophoresis of Steroid Conjugates

Abstract

Advances in medical sciences have mandated increased separation efficiency and high level sensitivity. This is especially true when sample availability is in the ultra micro range. Because of this a study of capillary electrophoresis (CE for separation of steroid conjugates was carried out. There are few methods available to separate these molecules without first hydrolysis of the conjugates. The initial focus was on the estrogen conjugates since many of these are readily available for reference material. We avoided the use of derivative formation often necessary for optimal sensitivity.