A direct difference infrared cell for studying the hydration of protein glasses
- 1 October 1982
- journal article
- Published by IOP Publishing in Journal of Physics E: Scientific Instruments
- Vol. 15 (10), 1073-1076
- https://doi.org/10.1088/0022-3735/15/10/028
Abstract
A method of obtaining direct difference infrared spectra of protein glasses is described. The cell constructions are given and the procedure for producing protein glasses outlined. The protein glasses are wedged shaped so as to allow easy adjustment of protein amounts in the spectrophotometer beams. The method is used to obtain difference spectra between wet (hydrated) and dry lysozyme glasses.Keywords
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