Regulation of methionine synthesis in Escherichia coli : Effect of metJ gene product and S -adenosylmethionine on the expression of the metF gene
Open Access
- 1 June 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (11), 3601-3605
- https://doi.org/10.1073/pnas.82.11.3601
Abstract
The regulation of the expression of the E. coli metF gene, which codes for 5,10-methylenetetrahydrofolate reductase (EC 1.1.99.15), was investigated by using a simplified DNA-directed in vitro system that measures the formation of the 1st dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription. S-Adenosylmethionine apparently is functioning as an allosteric effector.This publication has 28 references indexed in Scilit:
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