Thermodynamic and Kinetic Analysis of sCD4 Binding to HIV-1 Virions and of gp120 Dissociation
- 1 April 1992
- journal article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 8 (4), 443-450
- https://doi.org/10.1089/aid.1992.8.443
Abstract
Kinetic and thermodynamic aspects of the binding of sCD4 to intact virions of human immunodeficiency virus type 1 (HIV-1 RF), and of the subsequent induction of gp120 dissociation were studied. sCD4 binding to virions at 4 and 37 degrees C is half-maximal at approximately 40 and 10 nM, respectively. The transition between low-affinity and high-affinity binding of sCD4 to virions occurs over a narrow temperature range between 20 and 25 degrees C. Shedding of gp120 from virions after sCD4 binding is also temperature dependent, being initiated above approximately 20 degrees C. The minimum temperatures for the sCD4 affinity transition and gp120 shedding are, therefore, similar and we suggest how the two processes might be related mechanistically.Keywords
This publication has 53 references indexed in Scilit:
- Receptor-Mediated Activation of Immunodeficiency Viruses in Viral FusionScience, 1991
- ResponseScience, 1991
- Crystal structure of an HIV-binding recombinant fragment of human CD4Nature, 1990
- Morphometric Analysis of Recombinant Soluble CD4-Mediated Release of the Envelope Glycoprotein gp120 from HIV-1AIDS Research and Human Retroviruses, 1990
- Involvement of tryptase‐related cellular protease(s) in human immunodeficiency virus type 1 infectionFEBS Letters, 1989
- Highly efficient neutralization of HIV with recombinant CD4-immunoglobulin moleculesNature, 1989
- Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptorCell, 1987
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAVNature, 1984
- The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirusNature, 1984