Two dimensional diffusion theory: Cylindrical diffusion model applied to fluorescence quenching

Abstract

A value for the diffusion constant of fluorescent quenchers can be inferred by fitting measured and theoretical fluorescence decay curves. In this paper, a formalism is presented describing diffusion‐dependent fluorescence quenching in systems which are effectively two dimensional in nature. This model predicts somewhat different values for the diffusion constant from those one obtains by fitting the same experimental curve to the usual Smoluchowski model for diffusion in an isotropic (’’spherical’’) system.