A Dual Pathway for Acth Steroidogenic Action in Purified Adrenocortical Cells
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 4 (1-6), 301-313
- https://doi.org/10.3109/10799898409042557
Abstract
Isolated adrenal fasciculata cells were purified by centrifugation through a 0-50% hyperbolic gradient of PercollR. The dose-dependence and kinetics of both intracellular cyclic AMP accumulation and steroido-genesis in response to ACTH1-39 and ACTH5-24 (corticotropin-(1-39) and corticotropin-(5-24)-peptides) were determined using purified cells. The rate of intracellular cyclic AMP formation was maximal during the first five minutes after hormone addition and remained constant or fell thereafter. Therefore intracellular cyclic AMP accumulation, assessed after 5 min., was compared with steroid output after 20 min. Maximal steroidogenesis was elicited by ACTH5-24 without discerning a significant stimulation of intracellular cyclic AMP accumulation. ACTH6-24 (corticotropin-(6-24)-peptide) could completely inhibit the intracellular cyclic AMP accumulation elicited by ACTH1-39 or by ACTH5-24 at concentrations that only partially inhibited steroidogenesis. It is possible that there are two pathways for the steroidogenic action of ACTH, one of which is obligatorily mediated by intracellular cyclic AMP, and another which involves a different mediator.Keywords
This publication has 19 references indexed in Scilit:
- Phospholipid degradation and signal translation for protein phosphorylationTrends in Biochemical Sciences, 1983
- Mechanism of Action of ACTH in the Adrenal CortexPublished by Springer Nature ,1982
- Effects of ACTH (corticotropin) analogues on steroidogenesis and cyclic AMP in rat adrenocortical cells. Evidence for two different steroidogenically responsive receptorsBiochemical Journal, 1980
- Synthesis and Biological Activity of Four Adrenocorticotropin‐(1‐24)‐tetracosapeptide Analogues with N‐Terminal Deletions of Four to Seven Amino‐Acid ResiduesHelvetica Chimica Acta, 1978
- On the mechanism of action of cholera toxin on isolated rat adrenocortical cells Comparison with the effects of adrenocorticotropin on steroidogenesis and cyclic AMP outputBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- An investigation of the involvement of adenosine 3′:5′-cyclic monophosphate in steroidogenesis by using isolated adrenal cell column perfusionBiochemical Journal, 1975
- Steroidogenesis and Cyclic Adenosine 3′,5′-Monophosphate Accumulation in Rat Adrenal CellsPublished by Elsevier ,1973
- CORTICOSTEROIDOGENESIS IN ISOLATED ADRENAL CELLS: EFFECT OF ADRENOCORTICOTROPHIC HORMONE, ADENOSINE 3′,5′-MONOPHOSPHATE AND β1–24ADRENOCORTICOTROPHIC HORMONE DIAZOTIZED TO POLYACRYLAMIDEJournal of Endocrinology, 1972
- Radioimmunoassay for Cyclic NucleotidesJournal of Biological Chemistry, 1972
- Cyclic AMP and SteroidogenesisPublished by Elsevier ,1971