Expression of the human β-globin gene in mouse teratocarcinoma cells
- 1 June 1987
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 208 (1-2), 357-360
- https://doi.org/10.1007/bf00330466
Abstract
We have stably transformed PCC4 and F9 teratocarcinoma stem cells with the human β-globin gene. The transformed PCC4 cells retained their ability to produce in host mice tumours containing various differentiated tissues. Three of the five clones examined had human β-globin transcripts, with correct 5′ termini; however, some transcripts were also initiated upstream from the natural cap site in two of the three clones. The expression of the human β-globin gene was maintained when the stem cell phenotype was changed, either into endodermal cell type by retinoic acid treatment of the F9 clones, or into fibroblastic cell type by cell hybridization of transformed PCC4 cells with L fibroblasts. Thus, the human β-globin gene introduced into early embryonic cells can be expressed constitutively and its expression is maintained when the pattern of gene expression in the cells is changed.Keywords
This publication has 32 references indexed in Scilit:
- Persistence of transcription of the human β-globin gene in hybrids between transfected F9 embryonal carcinoma and L cellsCell Differentiation, 1984
- Regulated expression of an introduced MHC H–2Kbm1 gene in murine embryonal carcinoma cellsNature, 1984
- Tissue-specific activation of a cloned α-fetoprotein gene during differentiation of a transfected embryonal carcinoma cell lineNature, 1984
- Regulated expression of the human β-globin gene family in murine erythroleukaemia cellsNature, 1983
- The regulated expression of β-globin genes introduced into mouse erythroleukemia cellsCell, 1983
- Expression of a β-globin gene is enhanced by remote SV40 DNA sequencesCell, 1981
- Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNANature, 1979
- The induction of differentiation in teratocarcinoma stem cells by retinoic acidCell, 1978
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974