Bidirectional regulation of cAMP generating system by dopamine-D1 and D2-receptors in the rat retina

Abstract
Accumulation or inhibition of cAMP formation in response to dopamine or dopamine related drugs in the absence or in the presence of forskolin and/or IBMX was investigated in isolated rat retina. While the existence of D1-receptors (positively coupled with adenylate cyclase) was confirmed, D2-receptors (negatively coupled to adenylate cyclase) were also revealed by using a selective D1-antagonist (SCH 23390), a D2-agonist (LY 171555) or two D2)-antagonists (S-sulpiride, spiroperidol). These results indicate that rat retina may be used for the study of both types of dopamine-receptors.