NF-κB Subunit Regulation in Nontransformed CD4 + T Lymphocytes
- 5 June 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5062), 1452-1456
- https://doi.org/10.1126/science.1604322
Abstract
Regulation of interleukin-2 (IL-2) gene expression by the p50 and p65 subunits of the DNA binding protein NF-kappa B was studied in nontransformed CD4+ T lymphocyte clones. A homodimeric complex of the NF-kappa B p50 subunit was found in resting T cells. The amount of p50-p50 complex decreased after full antigenic stimulation, whereas the amount of the NF-kappa B p50-p65 heterodimer was increased. Increased expression of the IL-2 gene and activity of the IL-2 kappa B DNA binding site correlated with a decrease in the p50-p50 complex. Overexpression of p50 repressed IL-2 promoter expression. The switch from p50-p50 to p50-p65 complexes depended on a protein that caused sequestration of the p50-p50 complex in the nucleus.Keywords
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