A novel ADP- and zinc-binding fold from function-directed in vitro evolution
- 14 March 2004
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 11 (4), 382-383
- https://doi.org/10.1038/nsmb745
Abstract
A great challenge to biologists is to create proteins with novel folds and tailored functions. As an alternative to de novo protein design, we investigated the structure of a randomly generated protein targeted to bind ATP. The crystal structure reveals a novel alpha/beta fold bound to its ligand, representing both the first protein structure derived from in vitro evolution and the first nucleotide-binding protein stabilized by a zinc ion.Keywords
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