A grinding method for producing sections of large areas of plastic embedded tissues
- 1 September 1982
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 127 (3), 271-276
- https://doi.org/10.1111/j.1365-2818.1982.tb00422.x
Abstract
A method utilizing relatively thick ground sections of plastic-embedded tissue was developed, which affords the resolution obtained with 0.5-.mu.m cut sections. The sections, which are permanently affixed to plastic microscope slides, are much larger in area than ultramicrotome sections. Sections can be destained and restained and selected areas can be examined with various forms of EM. Autoradiographic studies are also possible. Although the method has a broader application, it is particularly useful in examining the interface between hard and soft tissues.Keywords
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