Control of growth by picolinic acid: Differential response of normal and transformed cells
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7), 2889-2893
- https://doi.org/10.1073/pnas.74.7.2889
Abstract
Picolinic acid reversibly inhibits the growth of cultured cells. Fourteen other pyridine derivatives were ineffective or toxic. Untransformed normal rat kidney (NRK) cells are reversibly arrested in the G1 stage of the growth cycle as shown by cell counts, mitotic index, [3H]thymidine incorporation and flow microfluorometry. Flow microfluorometry was used to monitor the effects of picolinic acid on numerous other cell lines. Normal cells are blocked in G1, whereas transformed cells show responses that are dependent on the transforming virus and independent of species or origin of the cell line. Kirsten sarcoma virus transformed cells are blocked in G1. SV 40 transformed cells progress to a G2 block. Cells transformed by polyoma or Harvey sarcoma virus with Moloney virus coat have flow microfluorometry profiles that indicate blocks in G1 and G2. Cells transformed with Moloney sarcoma virus are not blocked in a specific phase of the cell cycle. Picolinic acid does not change the levels of NAD+ plus NADH. Growth inhibition by picolinic acid is partially overcome by nicotinamide. These results suggest that picolinic acid interacts with a specific growth control mechanism that may involve NAD+ and this control mechanism is altered by different transforming viruses in different manners.This publication has 15 references indexed in Scilit:
- NRK cells synchronized in G1by picolinic acid are super-sensitive to prostaglandin E1stimulationFEBS Letters, 1977
- Control of growth of benzo(a)pyrene-transformed 3T3 cells.Proceedings of the National Academy of Sciences, 1976
- Mechanism of 3-mercaptopicolinic acid inhibition of hepatic phosphoenolpyruvate carboxykinase (GTP).Journal of Biological Chemistry, 1976
- Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.The Journal of cell biology, 1975
- Morphological changes in cultured mammalian cells: Prevention by the calcium ionophore A23187Cell, 1975
- Analogues of fusaric (5-butylpicolinic) acid as potent inhibitors of dopamine -hydroxylase.1973
- A23187: A Divalent Cation IonophoreJournal of Biological Chemistry, 1972
- Inhibition of DNA Synthesis in Animal Cells by Ethylene Diamine Tetraacetate, and Its Reversal by ZincProceedings of the National Academy of Sciences, 1972
- Cell Density-Dependent Changes of Glycolipid Concentrations in Fibroblasts, and Loss of This Response in Virus-Transformed CellsProceedings of the National Academy of Sciences, 1970
- STUDIES ON POLYMER OF ADENOSINE DIPHOSPHATE RIBOSE .I. ENZYMIC FORMATION FROM NICOTINAMIDE ADENINE DINUCLEOTIDE IN MAMMALIAN NUCLEI1967