Hexafluoroisopropyl Alcohol is a Useful Cosolvent with Dimethylformamide for Tryptic Synthesis of Peptides
- 1 February 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Chemistry Letters
- Vol. 21 (2), 327-330
- https://doi.org/10.1246/cl.1992.327
Abstract
A low-water-content solvent system, 4% H2O in hexafluoroisopropyl alcohol/dimethylformamide (1:1, v/v), was discovered for the efficient peptide synthesis catalyzed by trypsin. This mixed solvent retains potential superiority to the Klibanov’s solvent, 1% H2O and 9% formamide in t-amyl alcohol, in the synthesis of longer biologically active peptides by enzymatic condensation.Keywords
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