Resolution and Purification of Histories on Homologous Series of Hydrocarbon-Coated Agaroses
- 1 November 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 70 (1), 269-274
- https://doi.org/10.1111/j.1432-1033.1976.tb10978.x
Abstract
Hydrophobic chromatography on alkyl‐agarose columns has been applied to the fractionation of histones. This paper describes: (a) a two‐column method for the resolution of whole histone from calf thymus into its five main components (H1, H2a, H2b, H3 and H4), (b) a rapid one‐step procedure for the isolation of the H3 fraction from whole histone, (c) an alternative one‐step procedure for the resolution of H3 and H2a (which co‐elute during gel exclusion chromatography on Biogel P‐60). These experiments are also used for gaining further insight into the mechanism of action of hydrocarbon‐coated agaroses.This publication has 27 references indexed in Scilit:
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