Isolation and sequence of the pyridoxal 5'-phosphate active-site peptide from Rhodospirillum rubrum ribulose-1,5-bisphosphate carboxylase/oxygenase
- 14 October 1980
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 19 (21), 4848-4853
- https://doi.org/10.1021/bi00562a022
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase from R. rubrum was modified with pyridoxal 5''-phosphate and then reduced with sodium borohydride. Both carboxylase and oxygenase activities were lost when 1 molecule of pyridoxal 5''-phosphate was bound/enzyme dimer. Peptide maps of modified enzyme showed one N6-(phosphopyridoxal)lysine-containing peptide. This peptide was isolated by gel filtration and cation-exchange chromatography and its sequence determined as Ala-Leu-Gly-Arg-Pro-Glu-Val-Asp-(PLP-Lys)-Gly-Thr-Leu-Val-Ile-Lys. Since activation of the enzyme with Mg2+/CO2 enhances pyridoxal 5''-phosphate modification and subsequent inactivation and the substrate ribulose bisphosphate protects against modification, the modified lysyl group is most certainly at the catalytic site and not at the activation site of the enzyme.This publication has 23 references indexed in Scilit:
- Modification of Rhodospirillum rubrum ribulose bisphosphate carboxylase with pyridoxal phosphate. 1. Identification of a lysyl residue at the active siteBiochemistry, 1978
- Modification of ribulose bisphosphate carboxylase by 2,3-butadioneBiochemical and Biophysical Research Communications, 1978
- Sequences of two active-site peptides from spinach ribulosebisphosphate carboxylase/oxygenaseBiochemical and Biophysical Research Communications, 1978
- Reactivation of enzymes by light-stimulated cleavage of reduced pyridoxal 5'-phosphate-enzyme complexesBiochemistry, 1977
- Inactivation of ribulosebisphosphate carboxylase/oxygenase from spinach with the affinity label N-bromoacetylethanolamine phosphateBiochemical and Biophysical Research Communications, 1977
- Reaction of ribulosebisphosphate carboxylase from Rhodospirillum rubrum with the potential affinity label 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphateBiochemical and Biophysical Research Communications, 1977
- Essential primary amino groups of ribulose bisphosphate carboxylase/oxygenase indicated by reaction with pyridoxal 5′-phosphateArchives of Biochemistry and Biophysics, 1977
- Inhibition of D-ribulose 1,5-bisphosphate carboxylase by pyridoxal 5′-phosphateBiochemical and Biophysical Research Communications, 1976
- Spinach Ribulose Diphosphate Carboxylase. I. Purification and Properties of the Enzyme*Biochemistry, 1966
- Inhibition of Chymotrypsin Activity in Crystalline Trypsin PreparationsJournal of Biological Chemistry, 1964