Identification and typing of herpes simplex viruses with monoclonal antibodies

Abstract

Monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. Complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral DNA and by indirect immunofluorescence with monoclonal antibodies. Examination of a limited number of ulcerative lesions (human) revealed that indirect immunofluorescence and virus isolation were comparable in detecting herpes simplex virus. Thus, monoclonal antibodies evidently can be used to accurately identify and type isolates of herpes simplex virus.