The karyotypes of 12 species of Sarcophagidae are described in detail. These species are Wohlfahrtia meigeni (Schin.), Wohlfahrtia opaca (Coq.), Helicobia sp., and Helicobia rapax Walk., Neobellieria bullata (Park.), Sarcophaga exuberans Pand., Sarcophaga crassipalpis Macq. (including collections identified as Sarcophaga securifera Vill.), Sarcophaga argyrostoma R.D. (including Parasarcophaga argyrostoma Desv., Sarcophaga falculata Pand.), Hystricocnema plinthopyga (Wied.), Paraphrissopoda chrysostoma (Wied.), Euboettcheria sp., Sarcophaga occipitalis Thorns., Boettcheria cimbicis (Towns.), All of these species have six pairs of chromosomes. The heterochromatic X-chromosomes are usually acrocentric and vary greatly in length in different species. The heterochromatic Y-chromosomes are smaller than the corresponding X-chromosomes. Autosomal pairs of different species differ in relative length, arm ratios, and the locations of secondary constrictions. These, with other published descriptions, provide data on the karyotypes of a total of 24 species in this family. These accumulated chromosomal data are considered in relation to Roback's classification of the subfamily Sarcophaginae.