Nucleotide sequence of Escherichia coli K-12 replication origin.
- 1 February 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (2), 575-579
- https://doi.org/10.1073/pnas.76.2.575
Abstract
From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.This publication has 30 references indexed in Scilit:
- Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.Proceedings of the National Academy of Sciences, 1979
- Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.Proceedings of the National Academy of Sciences, 1978
- Structure of the orgin of DNA replication of bacteriophage fd.Proceedings of the National Academy of Sciences, 1978
- Origin of replication, oriC, of the Escherichia coli chromosome on specialized transducing phages λasnMolecular Genetics and Genomics, 1978
- Cloning and mapping of the replication origin of Escherichia coli.Proceedings of the National Academy of Sciences, 1977
- Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coliPlasmid, 1977
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977
- Origin of replication of colicin E1 plasmid DNA.Proceedings of the National Academy of Sciences, 1977
- DNA regions essential for the function of a bacteriophage fd promoterNucleic Acids Research, 1977
- Restriction endonuclease mapping and mutagenesis of the F sex factor replication regionMolecular Genetics and Genomics, 1977