The Preparation and some Properties of Purified Diphtheria Toxoid

Abstract

The active principle of toxic filtrates of Corynebacterium diphtheriae is confined to a very small fraction of the filtrates. By formalizing filtrates and treating with acetic acid, a highly active fraction is precipitated. The active fraction can be further purified by reprecipitation or by dialysis, and solutions 100 times or more purer than the original toxoid obtained. Purified toxoid is a very stable substance. The purified solutions are protein in nature and contain S and occasionally P.