Binding of the virion protein mediating alpha gene induction in herpes simplex virus 1-infected cells to its cis site requires cellular proteins.
- 1 October 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (20), 7061-7065
- https://doi.org/10.1073/pnas.84.20.7061
Abstract
In herpes simplex virus 1-infected cells, the transcription of .alpha. genes, the first set of genes to be expressed, is induced by a virion component, the .alpha.-trans-induction factor, and requires a cis site. Homologs of the cis site are present in the promoter-regulatory domains of all .alpha. genes and bind two cellular proteins designated as .alpha.H1 and .alpha.H2-.alpha.H3. We report that .alpha.-trans-induction factor, synthesized in vitro or present in nuclear extracts of infected cells, forms complexes with viral DNA fragments containing its cis-acting site only in the presence of cellular proteins and only under conditions that also enable the binding of the .alpha.H1 protein to the DNA. The induction of .alpha. genes by .alpha.-trans-induction factor appears, therefore, to be mediated by the interaction of the viral protein with cellular proteins at its cis-acting site.Keywords
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