Analysis of Chicken Ribosomal RNA Genes and Construction of Lambda Hybrids Containing Gene Fragments
- 8 April 1977
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 195-197
- https://doi.org/10.1126/science.557836
Abstract
The ribosomal RNA genes (ribosomal DNA) of chicken are present in approximately 200 copies and are cleaved into two fragments of molecular weight 5 X 10(6) and 12 to 14 X 10(6) by restriction endonuclease Eco RI. Recombinant phages have been constructed in vitro by joining the smaller fragment of ribosomal DNA and the outer arms of DNA from the vector lambdagtWES-lambdaC. In one of the recombinants, the coding strand of the cloned fragment is in the proper orientation for transcription with lambda "early" genes; in the other two the orientation is reversed, with the coding strand in the proper position for transcription with lambda "late" genes.Keywords
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