Immunogenic Properties of a Soluble Tumor-Specific Transplantation Antigen Induced by Simian Virus 40

Abstract

Soluble tumor-specific transplantation antigen was obtained from dissociated tumor cells of the ascites or tissue-cultured BALB/c lines (mKSA) transformed by simian virus 40. Immunogenic activity as assayed by tumor rejection was clearly recovered in 50% yield in the crude membrane preparation and in at least 5% yield in the crude soluble preparation. This activity was specific. After chromatography on Sephadex G-150, this activity was preserved. Contrary to other studies, we were unable to use in vitro assays of cytotoxicity to monitor solubilization and purification of this antigen.