Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligodeoxyribonucleotides
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (15), 6227-6245
- https://doi.org/10.1093/nar/14.15.6227
Abstract
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5′ terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.Keywords
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