Two-dimensional paper chromatography of urinary indoles and related substances
- 1 November 1956
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 64 (3), 481-485
- https://doi.org/10.1042/bj0640481
Abstract
Indoles and other metabolites of tryptophan can be conveniently separated on two-dimensional paper chromatograms with the organic layer of a butanol-acetic acid-water mixture (4:1:5, by volume) as first solvent and 20% (w/v) aqueous KC1 as 2nd solvent. The chromatographic behavior of nearly 50 compounds is described, and the chromatographic pattern of the principal substances related to tryptophan metabolism is summarized in a "map of the spots".Keywords
This publication has 13 references indexed in Scilit:
- The oxidation of tryptophan and some related compounds with persulphateBiochemical Journal, 1956
- INTERRELATIONSHIPS OF TRYPTOPHAN, NICOTINIC ACID AND OTHER B VITAMINSBritish Medical Bulletin, 1956
- A RAPID CHROMATOGRAPHIC TEST FOR HIGH URINARY EXCRETION OF 5-HYDROXY-INDOLE-ACETIC ACID AND 5-HYDROXYTRYPTAMINEThe Lancet, 1955
- PAPER CHROMATOGRAPHY OF URINARY INDOLESThe Lancet, 1955
- HUMORAL EFFECTS OF METASTASISING CARCINOID TUMOURSThe Lancet, 1955
- [Oxytryptamin (Serotonin) as a new hormone; with a special reference to its relation to the syndrome of metastatic carcinoids].1955
- Excretion of conjugated 2-amino-3-hydroxyacetophenone by man, and its significance in tryptophan metabolismBiochemical Journal, 1955
- Nonspecific formation of hydroxylated metabolites of the aromatic amino acidsArchives of Biochemistry and Biophysics, 1955
- On the excretion of indole derivatives in phenylketonuriaArchives of Biochemistry and Biophysics, 1954
- Chromatography in the study of amino-acid metabolism.1952