New system for cultivation of Neisseria gonorrhoeae.
- 1 April 1974
- journal article
- research article
- Vol. 27 (4), 802-5
Abstract
A modified Thayer-Martin medium, containing 2.0% agar, 0.25% dextrose, and 5.0 mug of trimethoprim per ml was compared to Thayer-Martin medium for growth of stock cultures of Neisseria gonorrhoeae and for isolation of N. gonorrhoeae from clinical specimens. Stock cultures and male urethral cultures grew equally well on both media, but the modified Thayer-Martin medium detected 10% more female patients positive for gonococci. A description is given of the use of a citric acid-sodium bicarbonate tablet for generating carbon dioxide in a sealed plastic bag containing a culture plate. Equivalent results were obtained on duplicate plates of 336 cultures and specimens when this procedure was compared with the standard candle jar extinction method of incubation. The tablet and plastic bag system has the advantages of economy, ease of use, and safety.This publication has 6 references indexed in Scilit:
- Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence.Sexually Transmitted Infections, 1972
- Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis.1971
- Primary isolation of N. gonorrhoeae with a new commercial medium.1967
- Further studies with a selective medium for cultivating Neisseria gonorrhoeae.Sexually Transmitted Infections, 1965
- A selective medium for the cultivation of N. gonorrhoeae and N. meningitidis1964
- STUDIES OF GONOCOCCAL INFECTION. I. A STUDY OF THE MODE OF DESTRUCTION OF THE GONOCOCCUS IN VITRO1JCI Insight, 1937