Amino‐Acid Sequences of the Active‐Site Sulfhydryl Peptide and Other Thiol Peptides from the Cysteine Proteinase α‐Clostripain
- 1 February 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 130 (3), 473-479
- https://doi.org/10.1111/j.1432-1033.1983.tb07174.x
Abstract
One free SH group in the heavy chain of .alpha.-clostripain [from Clostridium histolyticum] reacts rapidly with N-tosyllysine chloromethyl ketone which inactivates the enzyme. Iodoacetic acid also reacts with the thiol group required for enzyme activity but more slowly. A tryptic peptide containing the reactive sulfhydryl group labeled with iodo[1-14C]acetic acid was isolated and determined to be Gln-Ser-Val-Asp-Leu-Leu-Ala-Phe-Asp-Ala-Cys-Met. All others cysteine peptides were isolated from the trypsin hydrolysate of the [14C]carboxymethylated enzyme. N-terminal and C-terminal sequences of both chains of .alpha.-clostripain were determined. The sequences representing 20% of the primary structure of .alpha.-clostripain are not homologous with other cysteine proteinases or with any other protein structure known to date.Keywords
This publication has 16 references indexed in Scilit:
- Primary structure study of rat liver cathepsin B -----A striking resemblance to papainBiochemical and Biophysical Research Communications, 1980
- Structural studies on stem bromelain isolation, characterization and alignment of the cyanogen bromide fragmentsFEBS Letters, 1976
- A general procedure for the manual sequencing of small quantities of peptidesAnalytical Biochemistry, 1975
- Identifizierung von PTH-Aminosäuren mit Hilfe der schnellen Flüssigkeits-ChromatographieChromatographia, 1973
- 13 Papain, X-Ray StructurePublished by Elsevier ,1971
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- The covalent structure of a human γG-immunoglobulin. II. Isolation and characterization of the cyanogen bromide fragmentsBiochemistry, 1968
- A Protein SequenatorEuropean Journal of Biochemistry, 1967
- Amino acid sequence involving the reactive thiol group of ficinBiochemical and Biophysical Research Communications, 1964
- The activity of Clostridium histolyticum proteinase on synthetic substratesArchives of Biochemistry and Biophysics, 1953