Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y
- 1 January 1995
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 95 (1), 12-17
- https://doi.org/10.1007/bf00225066
Abstract
The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations.This publication has 13 references indexed in Scilit:
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