THE SENSITIVITY OF OOCYTES IN POST-NATAL RHESUS MONKEYS TO X-IRRADIATION
- 1 August 1966
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 12 (1), 183-+
- https://doi.org/10.1530/jrf.0.0120183
Abstract
Summary. Thirteen pre-pubertal and fifteen sexually mature rhesus monkeys (Macaca mulatta) were subjected to 1000 to 12,000 r unilateral X-irradiation; the contralateral ovary served as a control. The ovaries were removed at intervals of 6 hr to 4 weeks later, and the numbers of normal and atretic oocytes at each stage of follicular maturation were counted in every 40th section of both treated and control gonads. Doses of 4000 to 12,000 r induced cytoplasmic eosinophilia in oocytes, pyknosis in many granulosa cells and the destruction of most of the multi-layered follicles. The changes became more marked as the post-irradiation interval was extended. The numbers of primordial oocytes were unaffected by exposure to less than 3000 r; such oocytes were, however, almost totally eliminated 7 days after treatment with 12,000 r. The LD50/30 for germ cells at this developmental stage is about 5000 r. Oocytes enveloped by more than one layer of granulosa cells were more radio-sensitive, only a small proportion surviving for 4 days after exposure to 4000 r. Ovaries recovered 25 days following irradiation with 5000 to 12,000 r contained few multi-layered follicles ; most of the germ cells were atretic and the granulosa cells pyknotic. The primordial oocyte of the rhesus monkey is markedly resistant to radiation-induced cell death (following the terminology of Mandl, 1964), and contrasts sharply with the primordial oocytes of other mammalian species.This publication has 13 references indexed in Scilit:
- The ultrastructure of oogonia and oocytes in the foetal and neonatal ratProceedings of the Royal Society of London. B. Biological Sciences, 1962
- A quantitative study of the sensitivity of oocytes to X-irradiationProceedings of the Royal Society of London. B. Biological Sciences, 1959
- CHANGES IN THE MOUSE AFTER X-RAY STERILIZATIONJournal of Endocrinology, 1956
- THE REACTIVITY OF THE X-IRRADIATED OVARY OF THE RATJournal of Endocrinology, 1956
- Effects of a Single Session of Whole Body Irradiation in the Rhesus MonkeyThe British Journal of Radiology, 1956
- FURTHER OBSERVATIONS ON OOCYTE NUMBERS IN MATURE RHESUS MONKEYS (MACACA MULATTA)Journal of Endocrinology, 1954
- THE NUMBER OF OOCYTES IN THE MATURE RHESUS MONKEY (MACACA MULATTA)Journal of Endocrinology, 1951
- THE NUMBERS OF NORMAL AND ATRETIC OVA IN THE MATURE RATJournal of Endocrinology, 1950
- Irradiation of the ovaries of guinea‐pigs and its effect on the oestrous cycleJournal of Anatomy, 1931
- Changes in the ovary of the mouse following exposure to X-rays.—Part III. Irradiation of the non-parous adultProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1927