Cadmium-Resistant Pseudomonas putida Synthesizes Novel Cadmium Proteins
- 7 September 1984
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 225 (4666), 1043-1046
- https://doi.org/10.1126/science.225.4666.1043
Abstract
Three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from Pseudomonas putida growing in 3 mM cadmium. The three proteins were induced during different phases of growth, and the smallest (molecular weight 3600; 3 gram atoms of cadmium) was released into the medium when the cells lysed. The results of amino acid analyses and of ultraviolet, circular dichroism, electron paramagnetic resonance, and cadmium-113 nuclear magnetic resonance spectroscopy suggest a novel cadmium(II)-zinc(II)-copper(I) cluster structure for the major protein.Keywords
This publication has 10 references indexed in Scilit:
- Cadmium-113 Magnetic Resonance SpectroscopyScience, 1983
- The physiological function of metallothioneinTrends in Biochemical Sciences, 1982
- Evidence for site-selective metal binding in calf liver metallothionein.Journal of Biological Chemistry, 1982
- Energy-dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureusJournal of Bacteriology, 1981
- Reduced cadmium transport determined by a resistance plasmid in Staphylococcus aureusJournal of Bacteriology, 1981
- Copper metallothionein, a copper-binding protein from Neurospora crassaNature, 1980
- Investigation of the structure of metallothioneins by proton nuclear magnetic resonance spectroscopyBiochemistry, 1980
- Molecular mechanisms of accommodation in Escherichia coli to toxic levels of Cd2+Journal of Bacteriology, 1975
- [117] Chromatographic determination of amino acids by the use of automatic recording equipmentMethods in Enzymology, 1963
- Chromatographic determination of cystine as cysteic acidBiochemical Journal, 1954