Growth Requirements of Clostridium tetani

Abstract
Growth of a strain of Clostridium tetani requires, in addition to the usual inorganic elements, the following substances: an acid hydrolysate of protein, tryptophane, adenine or hypoxanthine, pantothenic acid, thiamine, riboflayine, "folic acid," and probably biotin. One or more additional factors may be essential. A high grade of toxin has been produced on such a medium, in which the folic acid is supplied in a liver extract concentrate.