Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
Open Access
- 1 January 1981
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 88 (1), 241-244
- https://doi.org/10.1083/jcb.88.1.241
Abstract
Binding of 125I-labeled insulin to the surface receptors of Cloudman S-91 mouse melanoma cells (CCL 53.1) was studied at various phases (M, G1, S, and G2) in the cell cycle. Insulin-binding activity was persistently present during the cell cycle but the highest activity was noted at the S-phase. The insulin once bound to the cell surface receptors at any phase of the cell cycle was internalized and degraded, presumably through a lysosomal pathway. Insulin-indexing activity of melanoma cells was not affected by melanocyte-stimulating hormone.This publication has 26 references indexed in Scilit:
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