Comparison of 3H-Diisopropyl Fluorophosphate and 32P-Diisopropyl Fluorophosphate for Neutrophil Kinetic Studies

Abstract

In order to evaluate the validity of ³H-diisopropyl fluorophosphate (DFP) as a label for neutrophil kinetic studies, the blood and skin chamber kinetics of autologous neutrophils doubly labeled with ³H-DFP and ³²P-DFP were determined in 6 normal subjects. Blood disappearance time and skin chamber accumulation of label was slightly but significantly decreased using ³H values, indicating mild elution of the isotope from the cells. The degree of elution was small in comparison to the previously observed rate of elution of ³²P-DFP and suggests that ³H-DFP is an acceptable alternative label.