New Types of Mutation Affecting Formation of Alkaline Phosphatase by Bacillus subtilis in Sporulation Conditions

Abstract

Summary: Mutations defining three new loci, sapA, sapB and phoS, were detected by their ability to overcome the phosphatase-negative phenotype of early-blocked asporogenous mutants in sporulation conditions. Synthesis of alkaline phosphatase by Bacillus subtilis is subject to ‘vegetative’ and ‘sporulation’ controls. The phoS mutations resulted in constitutive production of alkaline phosphatase and so could be altered in either the ‘vegetative’ or the ‘sporulation’ control system. The sapA and sapB mutations only affected alkaline phosphatase formation in sporulation conditions, and were considered to be sporulation specific. They rendered ‘sporulation’ alkaline phosphatase formation independent of all the spo mutations tested, and so independent of the control of the dependent sequence of spo locus expression; as the enzyme was not formed constitutively, it remained subject to some other sporulation control.