Two different strains of Melampsora lini (Pers.) Lév. were grown on media similar to those described by Turel (Can. J. Bot. 47: 821–823). Alterations in organic and inorganic constituents did not, in general, prevent growth. Peptone and yeast extract, singly or in combination, supported growth, but coconut milk did not. Maintenance of the ambient temperature below 17 °C during transfer procedures increased the number of colonies which grew. The mycelium was generally binucleate. Spore-like cells of varying shapes were found, some of which resembled uredospores and teliospores.