Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
- 1 July 2005
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 27 (13), 919-926
- https://doi.org/10.1007/s10529-005-7184-6
Abstract
Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6–48 mg PMP/g CDW) whilst highlighting population heterogeneity.Keywords
This publication has 13 references indexed in Scilit:
- Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometryJournal of Biotechnology, 2005
- Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processesBiotechnology Letters, 2004
- The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processesJournal of Industrial Microbiology & Biotechnology, 2004
- An industrial application of multiparameter flow cytometry: Assessment of cell physiological state and its application to the study of microbial fermentationsCytometry, 2001
- Current and future applications of flow cytometry in aquatic microbiologyFEMS Microbiology Reviews, 2000
- Bax-induced cell death in Pichia pastorisBiotechnology Letters, 1999
- Inclusion bodies and purification of proteins in biologically active formsPublished by Springer Nature ,1997
- Viability assessment of bacteria in mixed populations using flow cytometry*Journal of Microscopy, 1995
- Flow cytometry studies of recombinant Escherichia coli in batch and continuous cultures: DNA and RNA contents; light-scatter parametersApplied Microbiology and Biotechnology, 1994
- Characterization of bacteria by multiparameter flow cytometryJournal of Applied Bacteriology, 1992