Immunohistochemical localization and biochemical characterization of nerve growth factor receptor in adult rat brain
- 22 December 1989
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 290 (4), 585-598
- https://doi.org/10.1002/cne.902900411
Abstract
The expression of nerve growth factor (NGF) receptor in adult rat brain was studied by immunohistochemistry with a specific anti‐rat NGF receptor monoclonal antibody, 192‐IgG. Intense NGF receptor immunoreactivity (NGFRI) was found in structures known to be NGF responsive, including forebrain cholinergic neurons in medial septum, diagonal band of Broca, and basal nucleus of Meynert; central processes of neural‐crest‐derived sensory ganglion neurons and their innervated nucleus also contained such immunoreactivity. Distinct NGFRI staining was also found in many brain areas and cell types not known to be NGF responsive, including some hypothalamic regions, circumventricular organs, some areas related to the optic system, olfactory glomeruli, ependymal and subependymal cells in some locations, mesencephalic nucleus of the trigeminal nerve, cerebellar molecular layer, central linear nucleus, solitary tract and its nucleus, and inferior olive. The NGFRI in the circumventricular organs was further studied by in vivo labeling of 125I‐ligands. Intravenously injected 125I‐NGF, but not 125I‐cytochrome c, was specifically accumulated in the area postrema. Biochemical study of the NGF receptor showed a major band of molecular weight of approximately 90 KDa in the area postrema, choroid plexus, median eminence, and medial septum with the relative content consistent with that seen by immunohistochemistry. No evidence of a truncated NGF receptor was observed. The results of this study suggest that NGF and its receptor have broader roles in adult mammalian brain than previously thought.Keywords
This publication has 32 references indexed in Scilit:
- Retrograde transport of NGF by early chick embryo spinal cord motoneuronsDevelopmental Biology, 1988
- Transient expression of NGF-receptor-like immunoreactivity in postnatal rat brain and spinal cordBrain Research, 1988
- Basal forebrain magnocellular neurons stain for nerve growth factor receptor: Correlation with cholinergic cell bodies and effects of axotomyJournal of Neuroscience Research, 1987
- Placodal sensory neurons in culture: Nodose ganglion neurons are unresponsive to NGF, lack NGF receptors but are supported by a liver-derived neurotrophic factorDevelopmental Biology, 1985
- Characterization of the binding properties and retrograde axonal transport of a monoclonal antibody directed against the rat nerve growth factor receptor.The Journal of cell biology, 1985
- NGF-Mediated increase of choline acetyltransferase (ChAT) in the neonatal rat forebrain: Evidence for a physiological role of NGF in the brain?Developmental Brain Research, 1983
- Cholinergic innervation of cortex by the basal forebrain: Cytochemistry and cortical connections of the septal area, diagonal band nuclei, nucleus basalis (Substantia innominata), and hypothalamus in the rhesus monkeyJournal of Comparative Neurology, 1983
- The afferent connections of the suprachiasmatic nucleus of the golden hamster with emphasis on the retinohypothalamic projectionJournal of Comparative Neurology, 1982
- The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine‐B‐hydroxylase as a markerJournal of Comparative Neurology, 1975
- An autoradiographic study of the efferent connections of the ventral lateral geniculate nucleus in the albino rat and the catJournal of Comparative Neurology, 1974