The organisation of the mouse chromogranin B (secretogranin I) gene

Abstract

A cosmid clone containing the gene for mouse chromogranin B (secretogranin I), a secretory protein found in secretory granules of most endocrine cells and neurons, was isolated and sequenced. The chromogranin B protein was found to be encoded by 5 exons which correspond to the cleaved signal peptide, the short N‐terminal sequence preceding the disulfide‐bonded loop structure, the disulfide‐bonded loop structure itself, the large, variable region comprising ~90% of the protein, and the conserved C‐terminal sequence. The promoter region of the chromogranin B gene is very GC‐rich and contains a CATAA motif, a cAMP‐responsive element and an Spl binding site.