Evaluation of Methods for Preparing Proteins from Plasma High Density Lipoproteins

Abstract

The proteins from plasma high density lipoproteins have been prepared by three methods and the products are compared by electrophoresis on or in the media of paper, agarose, cellulose acetate, and polyacrylamide gel and by analytical ultracentrifugation. The capacities of the proteins to bind phospholipids, free fatty acids, and cholesterol are compared. The method based on that of Shore and Shore is the easiest to perform. The peptides are less likely to be denatured since no precipitation step is involved; this is supported by their generally greater affinity for lipids.