Studies on heterogeneity of human gastric zymogens
- 1 February 1970
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 116 (3), 397-404
- https://doi.org/10.1042/bj1160397
Abstract
The peptic zymogens of human gastric mucosa have been studied in the past by three different techniques: phosphate-gradient chromatography on a column of DEAE-cellulose, chloride-gradient chromatography on DEAE-cellulose and agar-gel electrophoresis. These techniques have given different results, each suggesting the presence of a different number of pepsin zymogens in human gastric mucosa. In the present experiments gastric mucosal homogenates were subjected to analysis by all three techniques. The two major zymogen peaks obtained from phosphate-gradient chromatography were found to be composed of the three fractions found on chloride-gradient chromatography; these fractions in turn were shown to be heterogeneous on agar-gel electrophoresis. The latter technique demonstrated the presence of seven separable enzymically active components in human gastric mucosal homogenates. The relationships of the components separated by the three techniques are discussed.Keywords
This publication has 15 references indexed in Scilit:
- Measurement of Pepsin I (Gastricsin) in Human Gastric JuiceGastroenterology, 1967
- Pepsinogen D. A fourth proteolytic zymogen from pig gastric mucosaBiochemical Journal, 1967
- COMPARATIVE STUDIES ON THE STRUCTURE AND SPECIFICITY OF HUMAN GASTRICSIN, PEPSIN AND ZYMOGENAnnals of the New York Academy of Sciences, 1967
- Pepsinogen C and pepsin C. Further purification and amino acid compositionBiochemical Journal, 1966
- PEPSINOGEN B: THE ZYMOGEN OF PEPSIN BBiochemical Journal, 1965
- Partial Characterization of Human Pepsin I, Pepsin IIA, Pepsin IIB, and Pepsin III*Biochemistry, 1964
- Purification and Properties of a Zymogen from Human Gastric MucosaJournal of Biological Chemistry, 1963
- Parapepsinogen II: the zymogen of parapepsin IIBiochemical Journal, 1960
- Chromatographic isolation of gastricsin, the proteolytic enzyme from gastric juice with pH optimum 3.2Biochimica et Biophysica Acta, 1958
- ENZYMIC DEPHOSPHORYLATION OF PEPSIN AND PEPSINOGENThe Journal of general physiology, 1958