Studies in Non-Protein Nitrogen. I. A Convenient Method for Measuring Urea in Blood

Abstract

The acid hydrolysis method of measuring urea has been revised so that it can be applied to either serum, plasma or whole blood. An acidified tungstic acid filtrate is subjected to a min. of 20 lb. of pressure for 90 min. in a pressure cooker or autoclave. The cooled soln. is neutralized and diluted, Gentzkow''s nesslerizing reagent is added, and the ammonia content is measured in a spectrophotometer at a wave length of 490 to 510 m[mu].