A PAPER CHROMATOGRAPHIC METHOD FOR THE MEASUREMENT OF PREGNANEDIOL IN URINE*

Abstract

A new method is described for the assay of urinary pregnanediol, employing enzymatic hydrolysis of pregnanediol glucuronide, ascending paper chromatography, and spectrophotometry. Analysis of absorption spectra, recovery experiments, and replicate assays of urine for pregnanediol establish the sensitivity, specificity and reliability of the method, which was also shown to reflect changing ovarian function during the menstrual cycle.